Bioinformatics Services Project Delivery Walkthrough
This page gives a detailed overview of how metagenomics projects are delivered to clients through Cosmos-Hub and how they can use it to gain actionable insights from their microbiome data generated by Cmbio.
| k-mer | nucleotide sequence of a certain length. It is common in genomics to select all possible kmers of a fixed length for each read in a sample. |
| WGS | whole genome shotgun sequencing - with this method of DNA sequencing, all microbial DNA in the sample is fragmented into small pieces for next-generation sequencing. |
| Shotgun Metagenomics | using wgs sequencing as described above, the Cosmos-Hub algorithms identify microorganisms based on the entire genomes of the organisms that are in our database |
| Amplicon/16S/ITS | unlike shotgun metagenomics, amplicon sequencing analysis looks only at the relevant conserved ribosomal RNA gene or genes, not the entire genome for identification. 16S rRNA gene is used for Bacterial identification, while ITS (internal transcribed spacer) is used for Fungi identification. |