CosmosID provides support for analyzing ZymoBIOMICS microbiome standards. With the purchase of these standards you are given CosmosID credits so you can interpret your results.

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How to Upload your Zymo Standard

To navigate to the Microbiome Standard page, click the Upload Microbiome Standard icon and you will see several Zymo standards to choose from.

Select the standard you purchased from Zymo. For more information about the standards, click “Learn more” below each standard.

Click “Select” to choose the Zymo standard you wish to upload and analyze.

Upload your sequence file by dragging and dropping it or by clicking on the window that says “Click to open file browser”.

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Sequence QC

After running your microbiome standard you will see a QC report for your sample.

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Length Distribution

Distribution of sequence lengths. In cases of untrimmed Illumina reads it will just contain a single peak. x: sequence length in base-pairs y: number of sequences featuring a particular length normalized to the total number of sequences

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GC-content

Distribution of GC-content. The GC-content of a sequence is calculated as the number of GC-bases compared to all bases (including ambiguous bases). x: relative GC-content of a sequence in percent y: number of sequences featuring particular GC-percentages normalized to the total number of sequences

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Ambiguous Base-content

Distribution of N-content. The N-content of a sequence is calculated as the number of ambiguous bases compared to all bases. x: relative N-content of a sequence in percent y: number of sequences featuring particular N-percentages normalized to the total number of sequences

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Quality Distribution

Distribution of average sequence quality scores. The quality of a sequence is calculated as the arithmetic mean of its base qualities. x: PHRED-score y: number of sequences observed at that quality score normalized to the total number of sequences

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Coverage

The number of sequences that support (cover) the individual base positions. In case of untrimmed Illumina reads it will just contain a rectangle. x: base position y: number of sequences covering individual base positions normalized to the total number of sequences

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Nucleotide Contributions

Coverage for the four DNA nucleotides and ambiguous bases. x: base position y: number of nucleotides observed per type normalized to the total number of nucleotides observed at that position

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Per-base GC Content

Combined coverage of G- and C-bases. x: base position y: number of G- and C-bases observed at current position normalized to the total number of bases observed at that position

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Quality Distribution