Amplicon Classification

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Introduction

Amplicon analysis refers to the identification of different organisms based on their taxonomy as identified by sequencing highly conserved regions of ribosomal RNA genes. For bacteria, the 16S gene is used for this identification and for fungi the ITS gene is used.

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How to Analyze 16S or ITS Samples with CosmosID

To analyze your 16S or ITS samples with CosmosID, just select either 16S amplicon or ITS amplicon when uploading the samples. Here is an example video using 16S samples:

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Interpreting 16S or ITS Results

The results for 16S and ITS are similar to those for wgs metagenomic analysis. When you click on a sample name from the datasets dashboard you will see a table with results. The columns include the Name, OTU ID, Frequency, and Relative Abundance.

Name: Organism identified at the taxonomy level you are viewing

OTU ID: Operational Taxonomic Unit identification. An OTU is a group of organisms as defined by DNA sequence similarity.

Frequency: The number of reads that matched the OTU.

Relative Abundance: The normalized abundance of the OTU based on frequency.

See View Results for more information about the visualizations available for 16S samples.