MaAsLin2 Results
Input Data
A pre-formatted .tsv table containing taxonomic or functional profiling results (values dependent on input parameters).Input Metadata
A pre-formatted .tsv table containing all sample metadata.Association Results
Perhaps the most important output from MaAsLin 2 is the list of significant associations displayed in the Association Results, which displays significant associations between metadata and microbial features along with effect size, p-values, and q-values. The full list of associations that pass MaAsLin 2’s significance threshold is ordered by increasing q-values. Columns are:- Metadata: the variable name being associated with a microbial feature.
- Feature Name: the microbial feature (taxon, gene, pathway, etc.).
- Value Name: for categorical features, the specific feature level for which the coefficient and significance of association is being reported.
- Coefficient/EffectSize: the model coefficient value
- Coefficients for categorical variables indicate the contrast between the each metadata category versus the reference.
- Standard Error: the standard error from the model.
- N: the total number of samples used in the model for this association (since minimum abundance/prevalence can exclude samples from comparisons).
- N not Zero: the total of number of these samples in which the feature is non-zero.
- P-value: the nominal significance of this association.
- Q-value: the corrected significance is computed with p.adjust with the correction method (BH, etc.).